goat anti-dat (Santa Cruz Biotechnology)
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90
Structured Review
Santa Cruz Biotechnology
goat anti-dat
Goat Anti Dat, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goat+anti+dat/goat+polyclonal+anti+dat+antibody++k+20/bio_rxiv__2025__03__21__644417-247-22-25
Average 90 stars, based on 1 article reviews
Goat Anti Dat, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goat+anti+dat/goat+polyclonal+anti+dat+antibody++k+20/bio_rxiv__2025__03__21__644417-247-22-25
Average 90 stars, based on 1 article reviews
goat anti-dat - by Bioz Stars,
2026-09
90/100 stars
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Incubation:Article Title: Inhibition of Nigral Microglial Activation Reduces Age-Related Loss of Dopaminergic Neurons and Motor Deficits Article Snippet: The free-floating 30-micrometer brain sections of interest were selected, washed with 0.3% Triton X-100 (X100, Sigma-Aldrich, St. Louis, MO, USA) in PBS, immersed in 3% H 2 O 2 to abolish endogenous peroxidase activity, and blocked with 3% normal goat serum (Cat# 005-000-001, RRID: AB_2336983, Jackson ImmunoResearch Inc., West Grove, PA, USA) to reduce non-specific bindings of antibody for 1 h at room temperature. .. The sections were then incubated with rabbit anti-tyrosine hydroxylase (TH) (1:2000; MAB152, Millipore, Darmstadt, Germany) for DA neurons, rabbit anti-ionized calcium-binding adapter molecule-1 (Iba1) (1:2000; 019-19741, Wako Pure Chemical Industries, Osaka, Japan) for microglia, and Article Title: [ 3 H]Dopamine Uptake through the Dopamine and Norepinephrine Transporters is Decreased in the Prefrontal Cortex of Transgenic Mice Expressing HIV-1 Transactivator of Transcription Protein Article Snippet: .. Samples were separated by 10% SDS-polyacrylamide gel electrophoresis for ∼90 minutes at 125 V. Samples were then transferred to Immobilon-P transfer membranes (0.45-μm pore size; Millipore Co., Bedford, MA) in transfer buffer (50 mM Tris, 250 mM glycine, 3.5 mM SDS) using a Mini Trans-Blot Electrophoretic Transfer Cell (Bio-Rad, Hercules, CA) for 90 minutes at 75 V. The membranes were then incubated with blocking buffer (5% milk powder in PBS containing 0.5% Tween-20) for 1 hour at room temperature, followed by incubation with either Article Title: Evidence for an Additive Neurorestorative Effect of Simultaneously Administered CDNF and GDNF in Hemiparkinsonian Rats: Implications for Different Mechanism of Action Article Snippet: .. After transfer of proteins to a nitrocellulose membrane, the membrane was incubated in 5% BSA for 1.5 h to block unspecific binding and then incubated in the presence of mouse anti-TH (1:5000, MAB 318, Millipore), Nucleic Acid Electrophoresis:Article Title: [ 3 H]Dopamine Uptake through the Dopamine and Norepinephrine Transporters is Decreased in the Prefrontal Cortex of Transgenic Mice Expressing HIV-1 Transactivator of Transcription Protein Article Snippet: .. Samples were separated by 10% SDS-polyacrylamide gel electrophoresis for ∼90 minutes at 125 V. Samples were then transferred to Immobilon-P transfer membranes (0.45-μm pore size; Millipore Co., Bedford, MA) in transfer buffer (50 mM Tris, 250 mM glycine, 3.5 mM SDS) using a Mini Trans-Blot Electrophoretic Transfer Cell (Bio-Rad, Hercules, CA) for 90 minutes at 75 V. The membranes were then incubated with blocking buffer (5% milk powder in PBS containing 0.5% Tween-20) for 1 hour at room temperature, followed by incubation with either Pore Size:Article Title: [ 3 H]Dopamine Uptake through the Dopamine and Norepinephrine Transporters is Decreased in the Prefrontal Cortex of Transgenic Mice Expressing HIV-1 Transactivator of Transcription Protein Article Snippet: .. Samples were separated by 10% SDS-polyacrylamide gel electrophoresis for ∼90 minutes at 125 V. Samples were then transferred to Immobilon-P transfer membranes (0.45-μm pore size; Millipore Co., Bedford, MA) in transfer buffer (50 mM Tris, 250 mM glycine, 3.5 mM SDS) using a Mini Trans-Blot Electrophoretic Transfer Cell (Bio-Rad, Hercules, CA) for 90 minutes at 75 V. The membranes were then incubated with blocking buffer (5% milk powder in PBS containing 0.5% Tween-20) for 1 hour at room temperature, followed by incubation with either Blocking Assay:Article Title: [ 3 H]Dopamine Uptake through the Dopamine and Norepinephrine Transporters is Decreased in the Prefrontal Cortex of Transgenic Mice Expressing HIV-1 Transactivator of Transcription Protein Article Snippet: .. Samples were separated by 10% SDS-polyacrylamide gel electrophoresis for ∼90 minutes at 125 V. Samples were then transferred to Immobilon-P transfer membranes (0.45-μm pore size; Millipore Co., Bedford, MA) in transfer buffer (50 mM Tris, 250 mM glycine, 3.5 mM SDS) using a Mini Trans-Blot Electrophoretic Transfer Cell (Bio-Rad, Hercules, CA) for 90 minutes at 75 V. The membranes were then incubated with blocking buffer (5% milk powder in PBS containing 0.5% Tween-20) for 1 hour at room temperature, followed by incubation with either Article Title: Evidence for an Additive Neurorestorative Effect of Simultaneously Administered CDNF and GDNF in Hemiparkinsonian Rats: Implications for Different Mechanism of Action Article Snippet: .. After transfer of proteins to a nitrocellulose membrane, the membrane was incubated in 5% BSA for 1.5 h to block unspecific binding and then incubated in the presence of mouse anti-TH (1:5000, MAB 318, Millipore), Membrane:Article Title: Evidence for an Additive Neurorestorative Effect of Simultaneously Administered CDNF and GDNF in Hemiparkinsonian Rats: Implications for Different Mechanism of Action Article Snippet: .. After transfer of proteins to a nitrocellulose membrane, the membrane was incubated in 5% BSA for 1.5 h to block unspecific binding and then incubated in the presence of mouse anti-TH (1:5000, MAB 318, Millipore), Binding Assay:Article Title: Evidence for an Additive Neurorestorative Effect of Simultaneously Administered CDNF and GDNF in Hemiparkinsonian Rats: Implications for Different Mechanism of Action Article Snippet: .. After transfer of proteins to a nitrocellulose membrane, the membrane was incubated in 5% BSA for 1.5 h to block unspecific binding and then incubated in the presence of mouse anti-TH (1:5000, MAB 318, Millipore), |